An animal model of trait anxiety: Carioca high freezing rats as a model of generalized anxiety disorder.

Despite being one of the main components of anxiety and playing a pivotal role in how an individual perceives and copes with anxiogenic situations or responds to a given treatment, trait anxiety is paradoxically omitted in most animal models of anxiety. This is problematic and particularly more concerning in models that are used to screen drugs and other treatments for specific anxiety disorders and to investigate their neurobiological mechanisms. Our group has been engaged in the search for specific anxiety-related traits in animal models of anxiety. We developed two new lines of rats with strong phenotypic divergence for high (Carioca High-conditioned Freezing [CHF]) and low (Carioca Low-conditioned Freezing [CLF]) trait anxiety as expressed in the contextual fear conditioning paradigm. Here, we summarize key behavioral, pharmacological, physiological, and neurobiological differences in one these lines, the CHF rat line, relative to randomized-cross controls and discuss how far they represent a valid and reliable animal model of generalized anxiety disorder and so high trait anxiety.

Molecular phylogeny and evolution of inflorescence types in Eperua.

PREMISE: The Amazonian hyperdominant genus Eperua (Fabaceae) currently holds 20 described species and has two strongly different inflorescence and flower types, with corresponding different pollination syndrome. The evolution of these vastly different inflorescence types within this genus was unknown and the main topic in this study. METHODS: We constructed a molecular phylogeny, based on the full nuclear ribosomal DNA and partial plastome, using Bayesian inference and maximum likelihood methods, to test whether the genus is monophyletic, whether all species are monophyletic and if the shift from bat to bee pollination (or vice versa) occurred once in this genus. RESULTS: All but two species are well supported by the nuclear ribosomal phylogeny. The plastome phylogeny, however, shows a strong geographic signal suggesting strong local hybridization or chloroplast capture, rendering chloroplast barcodes meaningless in this genus. CONCLUSIONS: With our data, we cannot fully resolve the backbone of the tree to clarify sister genera relationships and confirm monophyly of the genus Eperua. Within the genus, the shift from bat to bee and bee to bat pollination has occurred several times but, with the bee to bat not always leading to a pendant inflorescence.

EDITORIAL

Alice De Marchi Pereira de Souza, Amana Mattos, Edna Lúcia Tinoco Ponciano, Fábio Santos Bispo, Filipe Degani-Carneiro, Jimena de Garay Hernández, Laura Cristina de Toledo Quadros, Marcos Vinicius Brunhari, Michelle Menezes Wendling, Nazaré Albuquerque Hayasida, Patrícia Lorena Quitério, Rebeca Valadão Bussinger, Rodolfo Rodrigues de Souza, Rosana Lazaro Rapizo, Sabrina Dal Ongaro Savegnago, Vitor Castro Gomes

Traumatic aorta injuries in a rural area: late manifestations and review of therapeutic aspects.

Traumatic thoracic aortic injuries (TTAI) are associated with high rates of morbidity and mortality. They are classified according to the extent of damage and computed tomography angiography has the highest sensitivity and specificity for identifying the degree of injury and potential associated lesions. Treatment strategies for TTAI are based on the type and extent of injury and associated lesions. The patient's degree of stability can also help to define the choice of treatment, which can be conventional or endovascular surgery (EVAR) or even conservative management in selected cases. Among patients with adequate vascular anatomy, endovascular surgery is associated with better survival and fewer risks. The objective of this article is to describe a series of four cases followed up at a tertiary service in a Brazilian state that has few centers that provide high complexity care. Endovascular therapy was employed as the preferred method. All four patients had favorable outcomes, with no complications up to discharge, and are currently in outpatient follow-up.

Vestibulocochlear Delineation for Vestibular Schwannoma Treated With Radiation Therapy.

Purpose: To develop a specialist-based consensus of cochlear contouring to be used in patients undergoing stereotactic radiosurgery (SRS) treatment for vestibular schwannoma. Methods and Materials: Representative computed tomography (CT) and magnetic resonance imaging (MRI) were used for cochlear contouring. The semicircles, cochlea, vestibule, and internal acoustic meatus were delineated by 7 radiation oncology department physicians and reviewed by neuroradiologists. A total of 12 cases accrued from a single academic institution were studied for a similarity analysis by the Dice coefficient. Results: The suggested guideline is an easily reproductive tool that allows radiation oncologists to accurately contour the vestibulocochlear system to avoid toxicity due to inadequate dosimetry of organs at risk. This could be a useful tool even for non-vestibular schwannoma radiation therapy. The Dice coefficient suggests reproducible results as long as the following contouring recommendations are observed. Conclusions: The template for vestibulocochlear delineation may be useful for an adequate organs at risk definition. Future studies are required to find specific constraints for each segment of the vestibulocochlear system, and to mitigate interobserver variations.

Editorial / Editorial

Editorial da Revista Estudos e Pesquisas em Psicologia, v. 23, n. 1, p. 05-09 - Janeiro a Abril de 2023.

New geographic record in eastern Amazon Forest and potential distribution of Amphidecta calliomma (Lepidoptera: Nymphalidae).

Amphidecta calliomma is a butterfly species that occurs in Colombia, Bolivia, Peru, Venezuela, Ecuador, Panama, and Brazil (in the states of Mato Grosso, Mato Grosso do Sul, Rondônia, and Pará). Here, we present a new occurrence of A. calliomma in the Carajás National Forest (Pará, eastern Amazon), expanding the known distribution of the species. We also provide species distribution model comparing the contribution of the new occurrence to species area of occurrence projections, supporting future field research. The projections reveal an expansion of area of occurrence for A. calliomma located mainly in the southeast portion of Amazon Forest. Despite its wide distribution, the small number of records of A. calliomma may indicate that the species has a low detectability in surveys. This study provides support for new surveys and reduces the knowledge gap about A. calliomma, thus supporting its conservation.

Lesões traumáticas de aorta em zona rural: série de casos com manifestações tardias e revisão dos aspectos terapêuticos / Traumatic aorta injuries in a rural area: late manifestations and review of therapeutic aspects

Resumo As lesões traumáticas da aorta (LTA) torácica estão associadas a altas taxas de morbimortalidade. São classificadas de acordo com a extensão do dano, e a angiotomografia computadorizada tem as maiores sensibilidade e especificidade para identificar o grau de lesão e potenciais lesões associadas. As estratégias terapêuticas para LTA são baseadas no tipo de lesão, na extensão e nas lesões associadas. Pode auxiliar na definição de conduta também o grau de estabilidade do paciente, podendo ser manejo cirúrgico convencional, endovascular (TEVAR) ou conservador em casos selecionados. Entre os pacientes com anatomia vascular adequada, a cirurgia endovascular está associada a melhor sobrevida e a menos riscos. O objetivo deste artigo foi descrever uma série de quatro casos acompanhados em serviço terciário, em um estado com poucos serviços de alta complexidade. A terapêutica endovascular foi empregada como método preferencial. Os pacientes apresentaram evolução favorável sem complicações até a alta e encontram-se em acompanhamento ambulatorial.

Abstract Traumatic thoracic aortic injuries (TTAI) are associated with high rates of morbidity and mortality. They are classified according to the extent of damage and computed tomography angiography has the highest sensitivity and specificity for identifying the degree of injury and potential associated lesions. Treatment strategies for TTAI are based on the type and extent of injury and associated lesions. The patient's degree of stability can also help to define the choice of treatment, which can be conventional or endovascular surgery (EVAR) or even conservative management in selected cases. Among patients with adequate vascular anatomy, endovascular surgery is associated with better survival and fewer risks. The objective of this article is to describe a series of four cases followed up at a tertiary service in a Brazilian state that has few centers that provide high complexity care. Endovascular therapy was employed as the preferred method. All four patients had favorable outcomes, with no complications up to discharge, and are currently in outpatient follow-up.

Editorial / Editorial

Alice De Marchi Pereira de Souza, Edna Lúcia Tinoco Ponciano, Filipe Degani-Carneiro, Jimena de Garay Hernández, Laura Cristina de Toledo Quadros, Luiz Fernando Rangel Tura, Marcos Vinicius Brunhari, Patrícia Lorena Quitério, Rosana Lazaro Rapizo, Sabrina Dal Ongaro Savegnago, Vitor Castro Gomes

Editorial / Editorial

Editorial do volume 22, nº 1 (2022) da Revista Estudos e Pesquisas em Psicologia.

Escherichia coli and Pseudomonas aeruginosa Isolated From Urine of Healthy Bovine Have Potential as Emerging Human and Bovine Pathogens.

The study of livestock microbiota has immediate benefits for animal health as well as mitigating food contamination and emerging pathogens. While prior research has indicated the gastrointestinal tract of cattle as the source for many zoonoses, including Shiga-toxin producing Escherichia coli and antibiotic resistant bacteria, the bovine urinary tract microbiota has yet to be thoroughly investigated. Here, we describe 5 E. coli and 4 Pseudomonas aeruginosa strains isolated from urine of dairy Gyr cattle. While both species are typically associated with urinary tract infections and mastitis, all of the animals sampled were healthy. The bovine urinary strains were compared to E. coli and P. aeruginosa isolates from other bovine samples as well as human urinary samples. While the bovine urinary E. coli isolates had genomic similarity to isolates from the gastrointestinal tract of cattle and other agricultural animals, the bovine urinary P. aeruginosa strains were most similar to human isolates suggesting niche adaptation rather than host adaptation. Examination of prophages harbored by these bovine isolates revealed similarity with prophages within distantly related E. coli and P. aeruginosa isolates from the human urinary tract. This suggests that related urinary phages may persist and/or be shared between mammals. Future studies of the bovine urinary microbiota are needed to ascertain if E. coli and P. aeruginosa are resident members of this niche and/or possible sources for emerging pathogens in humans.

On the validity and taxonomic status of emCrypturellus/em emobsoletus/em emgriseiventris/em (Salvadori, 1895) and emC. o. hypochraceus/em (Miranda-Ribeiro, 1938) (Aves, Tinamidae).

The nine currently recognized subspecies in the Brown Tinamou (Crypturellus obsoletus) complex are disjunctly widespread in South America, and at least three of them occur in Brazil. Morphological diagnosis of most of these taxa is imprecise, in contrast with consistent vocal differences described in the literature. We conducted a taxonomic review of two Amazonian taxa, C. o. griseiventris and C. o. hypochraceus, using morphological, morphometric, and vocal characters. Our results indicate that C. o. hypochraceus (Miranda-Ribeiro, 1938) is a junior synonym of C. o. griseiventris (Salvadori, 1895), and that Crypturellus griseiventris (Salvadori, 1895) must be treated as a full species, based on unique and fully diagnosable plumage and vocal patterns.

Influence of hemodialysis blood flow rate on the thrombogenic potential in patients with central venous catheters.

In this study we apply methods to determine the tendency for thrombus formation in different central venous catheters (CVC) models associated with flow rate variation. To calculate the thrombogenic potential, we proposed a new numerical model of the platelet lysis index (PLI) equation. To compare the results of PLI and flow rate in different models of catheters, numerical calculations were performed on three different tips of CVC. The results showed that the PLI increases as a power function of the flow rate independent of the type of CVC. This study evidenced that the higher the blood flow rate used in the catheter, the greater the potential for thrombus formation. The PLI computed at the catheter outlet presented higher values when compared to the values computed at the vein outlet indicating that the blood flow through the CVC arterial lumen presents a proportionally larger thrombogenic potential when compared to the blood flow that leaves the vein towards the atrium. This finding may have consequences for clinical practice, since there is no specific flow value recommended in the catheter when the hemodialysis machine is turned on, and with this equation it was possible to demonstrate the thrombogenic potential that the flow rate can possibly offer.

Emergency Percutaneous Treatment of Saphenous Vein Graft Perforation After Cardiac Surgery.

Immediately post coronary artery bypass graft surgery, a 58-year-old woman developed excessive bleeding through the chest drains and hemodynamic instability, followed by total atrioventricular block requiring use of temporary pacemaker, which precluded electrocardiographic interpretation. She was referred for urgent diagnostic coronary angiography, which demonstrated important contrast leakage from the distal body of the saphenous vein graft to the first marginal branch. Acute saphenous vein graft perforation following coronary artery bypass graft surgery is rare. To the best of our knowledge, this is the first case report of acute saphenous vein graft perforation after coronary artery bypass graft surgery treated with a stent-graft.

COVID-19 Mortality Underreporting in Brazil: Analysis of Data From Government Internet Portals.

BACKGROUND: In Brazil, a substantial number of coronavirus disease (COVID-19) cases and deaths have been reported. It has become the second most affected country worldwide, as of June 9, 2020. Official Brazilian government sources present contradictory data on the impact of the disease; thus, it is possible that the actual number of infected individuals and deaths in Brazil is far larger than those officially reported. It is very likely that the actual spread of the disease has been underestimated. OBJECTIVE: This study investigates the underreporting of cases and deaths related to COVID-19 in the most affected cities in Brazil, based on public data available from official Brazilian government internet portals, to identify the actual impact of the pandemic. METHODS: We used data from historical deaths due to respiratory problems and other natural causes from two public portals: DATASUS (Department of Informatics of the Unified Healthcare System) (2010-2018) and the Brazilian Transparency Portal of Civil Registry (2019-2020). These data were used to build time-series models (modular regressions) to predict the expected mortality patterns for 2020. The forecasts were used to estimate the possible number of deaths that were incorrectly registered during the pandemic and posted on government internet portals in the most affected cities in the country. RESULTS: Our model found a significant difference between the real and expected values. The number of deaths due to severe acute respiratory syndrome (SARS) was considerably higher in all cities, with increases between 493% and 5820%. This sudden increase may be associated with errors in reporting. An average underreporting of 40.68% (range 25.9%-62.7%) is estimated for COVID-19-related deaths. CONCLUSIONS: The significant rates of underreporting of deaths analyzed in our study demonstrate that officially released numbers are much lower than actual numbers, making it impossible for the authorities to implement a more effective pandemic response. Based on analyses carried out using different fatality rates, it can be inferred that Brazil's epidemic is worsening, and the actual number of infectees could already be between 1 to 5.4 million.

Protocolo COVID-19 para higienização de profissionais de saúde no domicílio / Covid-19 protocol for hygienizing healthcare professionals at home

Objetivo: desenvolver um protocolo de higienização de profissional de saúde no domicílio, a fim de padronizar o processo e desenvolver uma lista de checagem (checklist) das etapas a serem realizadas pelo profissional de saúde para sua descontaminação baseada no protocolo desenvolvido. Método: Pesquisa de produção tecnológica, com enfoque em inovação. Utilizou-se da metodologia exploratória descritiva para realizar busca pelos temas Prevenção da COVID-19; Boas práticas de higienização; e Métodos de desinfecção, além das pesquisas sobre enfrentamento ao COVID-19. Essas produções foram analisadas e tornaram-se base teórica para desenvolvimento de um protocolo e checklist de higienização. Resultado: Obteve-se um protocolo sintetizado em um checklist de 19 passos capaz de reduzir a transmissão do vírus SARS-COV2 dos profissionais de saúde para os ambientes domiciliares e seus contactantes domésticos. Este protocolo se mostrou útil, não só a ser realizado pelos profissionais de saúde, como também aos profissionais expostos a COVID-19 em outros ambientes e até à população em geral. Conclusão: As etapas do protocolo e suas recomendações são simples de serem seguidas, assim como seus insumos são de fácil aquisição. Este protocolo se mostrou útil, não só a ser realizado pelos profissionais de saúde, como também aos profissionais expostos a COVID-19 em outros ambientes e até a população em geral. (AU)

Objective: to develop a health professional hygiene protocol at home, in order to standardize the process and develop a checklist of the steps to be performed by the health professional for their decontamination based on the developed protocol. Method: Technological production research, focusing on innovation. The descriptive exploratory methodology was used to search for the COVID-19 Prevention themes; Good hygiene practices; and Disinfection methods, in addition to the research on coping with COVID-19. These productions were analyzed and became a theoretical basis for the development of a hygiene checklist and protocol. Result: A protocol synthesized in a 19-step checklist was obtained, capable of reducing the transmission of the SARS-COV2 virus from health professionals to the home environment and their domestic contacts. This protocol proved to be useful, not only to be performed by health professionals, but also to professionals exposed to COVID-19 in other environments and even to the population in general. Conclusion: The steps of the protocol and its recommendations are simple to follow, as well as its inputs are easy to acquire. This protocol proved to be useful, not only to be performed by health professionals, but also to professionals exposed to COVID-19 in other environments and even the population in general. (AU)

Objetivo: desarrollar un protocolo de higiene de profesionales de la salud en el hogar, para estandarizar el proceso y desarrollar una lista de verificación de los pasos que debe realizar el profesional de la salud para su descontaminación con base en el protocolo desarrollado. Método: Investigación de producción tecnológica, centrada en la innovación. La metodología exploratoria descriptiva se utilizó para buscar los temas de Prevención de COVID-19; Buenas prácticas de higiene; y Métodos de desinfección, además de la investigación sobre cómo hacer frente a COVID-19. Estas producciones fueron analizadas y se convirtieron en una base teórica para el desarrollo de una lista de verificación y protocolo de higiene. Resultado: se obtuvo un protocolo sintetizado en una lista de verificación de 19 pasos, capaz de reducir la transmisión del virus SARS-COV2 de los profesionales de la salud al entorno doméstico y sus contactos domésticos. Este protocolo demostró ser útil, no solo para ser realizado por profesionales de la salud, sino también por profesionales expuestos a COVID-19 en otros entornos e incluso a la población en general. Conclusión: los pasos del protocolo y sus recomendaciones son fáciles de seguir, así como sus entradas son fáciles de adquirir. Este protocolo demostró ser útil, no solo para ser realizado por profesionales de la salud, sino también por profesionales expuestos a COVID-19 en otros entornos e incluso a la población en general. (AU)

Species Distribution Modelling: Contrasting presence-only models with plot abundance data.

Species distribution models (SDMs) are widely used in ecology and conservation. Presence-only SDMs such as MaxEnt frequently use natural history collections (NHCs) as occurrence data, given their huge numbers and accessibility. NHCs are often spatially biased which may generate inaccuracies in SDMs. Here, we test how the distribution of NHCs and MaxEnt predictions relates to a spatial abundance model, based on a large plot dataset for Amazonian tree species, using inverse distance weighting (IDW). We also propose a new pipeline to deal with inconsistencies in NHCs and to limit the area of occupancy of the species. We found a significant but weak positive relationship between the distribution of NHCs and IDW for 66% of the species. The relationship between SDMs and IDW was also significant but weakly positive for 95% of the species, and sensitivity for both analyses was high. Furthermore, the pipeline removed half of the NHCs records. Presence-only SDM applications should consider this limitation, especially for large biodiversity assessments projects, when they are automatically generated without subsequent checking. Our pipeline provides a conservative estimate of a species' area of occupancy, within an area slightly larger than its extent of occurrence, compatible to e.g. IUCN red list assessments.

Behavioral Effects of Systemic, Infralimbic and Prelimbic Injections of a Serotonin 5-HT2A Antagonist in Carioca High- and Low-Conditioned Freezing Rats.

The role of serotonin (5-hydroxytryptamine [5-HT]) and 5-HT2A receptors in anxiety has been extensively studied, mostly without considering individual differences in trait anxiety. Our laboratory developed two lines of animals that are bred for high and low freezing responses to contextual cues that are previously associated with footshock (Carioca High-conditioned Freezing [CHF] and Carioca Low-conditioned Freezing [CLF]). The present study investigated whether ketanserin, a preferential 5-HT2A receptor blocker, exerts distinct anxiety-like profiles in these two lines of animals. In the first experiment, the animals received a systemic injection of ketanserin and were exposed to the elevated plus maze (EPM). In the second experiment, these two lines of animals received microinjections of ketanserin in the infralimbic (IL) and prelimbic (PL) cortices and were exposed to either the EPM or a contextual fear conditioning paradigm. The two rat lines exhibited bidirectional effects on anxiety-like behavior in the EPM and opposite responses to ketanserin. Both systemic and intra-IL cortex injections of ketanserin exerted anxiolytic-like effects in CHF rats but anxiogenic-like effects in CLF rats. Microinjections of ketanserin in the PL cortex also exerted anxiolytic-like effects in CHF rats but had no effect in CLF rats. These results suggest that the behavioral effects of 5-HT2A receptor antagonism might depend on genetic variability associated with baseline reactions to threatening situations and 5-HT2A receptor expression in the IL and PL cortices. Highlights -CHF and CLF rats are two bidirectional lines that are based on contextual fear conditioning.-CHF rats have a more "anxious" phenotype than CLF rats in the EPM.-The 5-HT2A receptor antagonist ketanserin had opposite behavioral effects in CHF and CLF rats.-Systemic and IL injections either decreased (CHF) or increased (CLF) anxiety-like behavior.-PL injections either decreased (CHF) anxiety-like behavior or had no effect (CLF).